ABSTRACT
The chromatographic fingerprint was established by eluting with the mobile phase consisted of acetonitrile and 0.2% formic acid water on an Agilent TC-C18 (2) column (4.6 mm x 250 mm, 5 microm). Six chromatographic peaks were identified by HPLC-MS/MS method. Ten batches of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle were determined, and the similarity was arranged from 0.72 to 0.99. Good precision, stability and repeatability were obtained, and this study provides a reference for the quality control of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle.
Subject(s)
China , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Glycyrrhiza uralensis , Chemistry , Mass Spectrometry , Quality Control , Rhizome , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To establish HPLC fingerprints of Liuwei Dihuang condensed pills.</p><p><b>METHOD</b>Dikma Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with acetonitrile (containing 0.05% phosphoric) -water (containing 0.05% phosphoric) as the mobile phase. The column temperature was set at 40 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was 276 nm (0-10 min), 236 nm (10-40 min) and 276 nm (40-60 min). The sample size was 20 microL. Chromatographic peaks were identified by Q-TOF-MS-IDA-MS/MS method.</p><p><b>RESULT</b>Good precision, stability and repeatability were proved. Q-TOF-MS-IDA-MS/ MS method was adopted for qualitative determination of eighteen chromatographic peaks. Ten batches of Liuwei Dihuang condensed pills were determined with the method, and their similarities were above 0. 96.</p><p><b>CONCLUSION</b>The study lays a foundation for the overall quality evaluation of Liuwei Dihuang condensed pills.</p>
Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Reference Standards , Quality Control , TabletsABSTRACT
<p><b>OBJECTIVE</b>To study chemical constituents of glucoside fraction from Liuwei Dihuang Gantang and clarify its substance foundation of active constituents.</p><p><b>METHOD</b>Glucoside fraction was prepared by macroporous resin chromatography. Constituents were separated by silica gel and reverse phase silica gel column chromatography, and their structures were identified by MS and NMR.</p><p><b>RESULT</b>Eleven compounds were separated and identified as 7-dehydrologanin (1), 7alpha-O-methylmorroniside (2), 7beta-O-methylmorroniside (3), 7alpha-O-ethylmorroniside (4), 7beta-O-ethylmorroniside (5), morroniside (6), sweroside (7), loganin (8), paeoniflorin (9), paeonolide (10) and loganic acid (11).</p><p><b>CONCLUSION</b>All of those compounds were separated from Liuwei Dihuang Gantang for the first time.</p>
Subject(s)
Drugs, Chinese Herbal , Chemistry , Glucosides , Chemistry , Molecular StructureABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of eleutheroside B in As1 (the extract fraction from Acanthopanax senticosus, which has a good effect of antifatigue).</p><p><b>METHOD</b>The antifatigue effect of Asl was evaluated by mice burden swimming testing. One compound was isolated by silica gel, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography. The structure was identified by physicochemical properties and spectral evidences. Eleutheroside B in Asl was determined by HPLC. Chromatographic conditions included Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) and the mobile phase consisting of a mixture of acetonitrile-water-ethanoic acid (10: 90: 0.01). The UV detection wavelength was set at 344 nm.</p><p><b>RESULT</b>As1 showed an excellent antifatigue activity; One compound was isolated from As1 and its structure was identified as Eleutheroside B; The calibration cure was linear in the range of 0.104-20.8 microg (r = 0.9999), the average recovery was 97.68%, RSD 1.4% (n=6).</p><p><b>CONCLUSION</b>This HPLC method is simple,accurate and reliable.</p>
Subject(s)
Animals , Humans , Male , Mice , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Chemistry , Eleutherococcus , Chemistry , Fatigue , Drug Therapy , Glucosides , Phenylpropionates , SwimmingABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of an active fraction (DSS-A-N-30) from Danggui Shaoyao San.</p><p><b>METHOD</b>DSS-A-N30 was prepared by macroporous resin chromatography, the compound was isolated by column chromatography on silica gel and RPC-18, the structure was elucidated by spectroscopic methods.</p><p><b>RESULT</b>A new monoterpene glycoside was isolated and identified from DSS-A-N-30.</p><p><b>CONCLUSION</b>The new monoterpene glycoside was identified as 4"-hydroxyl-albiflorin.</p>
Subject(s)
Bridged-Ring Compounds , Chromatography, Gel , Drugs, Chinese Herbal , Chemistry , Magnetic Resonance Spectroscopy , MonoterpenesABSTRACT
<p><b>OBJECTIVE</b>To search the anti-inflammatory fraction of Albizia julibrissin.</p><p><b>METHOD</b>Inflammatory model of Kunming mice ear edema induced by croton oil and determination combined with the LC-MS-MS-guided fractionation and isolation were used.</p><p><b>RESULT</b>The n-butanol fraction (AJ-B) obtained from the ethanolic extract of the Cortex albiziae was the major active fraction. The lignan glycosides fraction (AJ-B-1), which was further isolated from AJ-B, showed significant anti-inflammatory activity and exhibited dose-dependent relationship in the dose of 5 to 20 mg x kg(-1).</p><p><b>CONCLUSION</b>The method of bioassay-guided fractionation and isolation combined with the LC-MS-MS determination may be of benefit to the logical studies on the bioactive fractions or constituents of traditional Chinese materia medica.</p>
Subject(s)
Animals , Male , Mice , Albizzia , Chemistry , Anti-Inflammatory Agents, Non-Steroidal , Therapeutic Uses , Biological Assay , Methods , Butanols , Chromatography, High Pressure Liquid , Methods , Croton Oil , Drugs, Chinese Herbal , Therapeutic Uses , Edema , Drug Therapy , Glycosides , Therapeutic Uses , Lignans , Therapeutic Uses , Phytotherapy , Plant Bark , Chemistry , Plants, Medicinal , Chemistry , Tandem Mass Spectrometry , MethodsABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Stellaria media.</p><p><b>METHOD</b>The compounds were isolated with various column chromatography and semi-preparative HPLC. The structures were determined by modem spectroscopic methods (IR, UV, 1H-NMR, 13C-NMR, HMBC, ESI-MS).</p><p><b>RESULT</b>Five compounds were isolated and identified as apigenin 6-C-beta-D-galactopyranosyl-8-C-alpha-L-arabinopyranoside (1), apigenin 6-C-alpha-L-arabinopyranosyl-8-C-beta-D-galactopyranoside (2), apigenin 6-C-beta-D-galactopyranosyl-8-C-beta-L-arabinopyranoside (3), apigenin 6-C-beta-D-glucopyranosyl-8-C-beta-D-galactopyranoside (4), apigenin 6, 8-di-C-alpha-L-arabinopyranoside (5).</p><p><b>CONCLUSION</b>Compounds 1-5 were obtained from Stellaria genus for the first time.</p>
Subject(s)
Apigenin , Chemistry , Chromatography, High Pressure Liquid , Methods , Flavones , Chemistry , Galactosides , Chemistry , Plants, Medicinal , Chemistry , Stellaria , ChemistryABSTRACT
As a traditional Chinese medicine, Danggui Shaoyao powder has been studied for more than one thousand years. This article reviews the recent advances in chemical constituents, pharmacological activities and clinical application.
Subject(s)
Animals , Humans , Central Nervous System , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Hypothalamo-Hypophyseal System , Medicine, Chinese Traditional , Pituitary-Adrenal System , Plants, Medicinal , Chemistry , PowdersABSTRACT
Cortex Albizziae, the stem bark of the leguminous plant Albizzia julibrissin, is specified in Chinese pharmacopoeia as a traditional Chinese medicine used to relieve melancholia and uneasiness of body and mind, invigorate the circulation of blood and subside a swelling. This article reviews the recent advances in chemical constituents and pharmacological activities of Cortex Albizziae.
Subject(s)
Animals , Humans , Albizzia , Chemistry , Antineoplastic Agents, Phytogenic , Pharmacology , Flavones , Chemistry , Hypnotics and Sedatives , Pharmacology , Molecular Structure , Plant Bark , Chemistry , Plants, Medicinal , Chemistry , Reproductive Control Agents , Pharmacology , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To establish a RP-HPLC method for determination of glycosides in Traditional Chinese Medicine Liu-wei Di-huang.</p><p><b>METHOD</b>The samples were analyzed on an ODS column at 30 degrees C, with mobile phase of methanol/water (33:67) at flow rate 1.0 mL.min and detection at wavelength of 236 nm.</p><p><b>RESULT</b>Three major components reached base-line separation and were identified to be mononiside, loganin, paeoniflorin. Respectively for the three components, linear correlations were found between peak areas and concentrations in the ranges of 7.4-60, 7.7-62 mg.L-1 and 8.5-68 mg.L-1, and the recoveries were 98.8%, 98.3%, 99.6%.</p><p><b>CONCLUSION</b>The established method is proved to be suitable for simultaneous quantification of three major glycosidic components in Liuwei Dihuang decoction and can be used for evaluation of the quality of Liuwei Dihuang preparations.</p>